Translational_Unit

Part:BBa_K371007:Design

Designed by: Yang Zhang   Group: iGEM10_USTC   (2010-08-27)


pduAB+pduJK+pduN+pduTUV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3028
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because the natural sequence of pdu A, pduB and pduJ has a PstI restriction site. Site-mutation method has been applied to remove thess site to meet the RFC10 standard. The sequencing result of some gene shows some silent mutation when compare with the reference sequence we got from NCBI. But these are also few error mutation in pduK and pduTUV. We think the difference between our result and the reference come deprive from the devergence of bacterial strain. We are still do experiment do certify that our system can work well.


Source

All these genes come from the strain of Citrobacter Freundi(NBRC NO.12681) by PCR

References